Introducing HBDH

HOW IT WORKS

Helping you create tests that measure what matters most.

Using cutting edge recombinant technology, our Hydroxybutyrate Dehydrogenase (HBDH) works by measuring ß-Hydroxybutyrate, the most abundant ketone that builds up during ketosis.

This is unlike traditional nitroprusside methods, which only react with acetoacetate and acetone. By using HBDH, you are able to detect DKA earlier and more accurately than ever before.

ß-Hydroxybutyrate

Acetoacetate (20%)

Acetone (2%)

With the silent epidemic of diabetes on the rise, the urgency to address diabetic ketoacidosis (DKA) cannot be overstated.

As a world leading manufacturer of critical materials for the diabetes market, BBI Solutions is proud to launch our new recombinant HBDH to address this global threat.

Proven Reliability

Our recombinant manufacturing process delivers best in class consistency, so you can trust in the efficacy of our HBDH in detecting DKA.

Consistent quality

As an ISO 13485 certified manufacturer you can rest assured you have access to a consistent high quality product, batch after batch, saving you time and resources.

5 Billion blood glucose test strips and counting

As the trusted component in over 5 billion tests for the most demanding, world-leading IVD manufacturers, our track record speaks for itself - as does our extensive portfolio of diabetes products.

From raw materials to results

As a global leader in the primary manufacture of diagnostic enzymes, we are a key player in the supply of critical raw materials for the diagnosis and management of diabetes.

Wraparound support as standard

Our experts support you in building customised assays and tests, ensuring the market success of your diagnostic solutions.

Investing in diabetes

Our new Centre of Excellence strengthens our recombinant manufacturing capabilities to fully cater to our customers and deliver a wider range of critical raw materials.

testimonial

BBI has a long-standing commitment to diabetes management and we’re excited to add HBDH to our extensive range.

Customers will benefit from our new state-of-the-art Centre of Excellence which expands and upgrades our ability to cultivate and purify recombinant proteins like HBDH.

This is supported, as always, by our team of world-leading experts at every stage, giving BBI customers an advantage in developing best-in-class end products.

Mario Gualano

CEO, BBI Solutions

SUpport

Frequently Asked Questions

Tech specs

D-3-Hydroxybutyrate Dehydrogenase (HBDH)

Alternative names

(R)-3- Hydroxybutanoate : NAD+ oxidoreductase

Product code

rHBDH

E.C number

1.1.1.30

CAS number

9028-38-0

Systematic name

NAD+ oxidoreductase

Source

Recombinant HBDH (Alcaligenes faecalis) expressed in E. Coli

Form

A freeze-dried material

Suggested applications

Recombinant enzyme to measure serum levels of ß-Hydroxybutyrate as a method to diagnose and monitor diabetic ketoacidosis (DKA) and its treatment.

Enzyme function

ß-Hydroxybutyrate is measured with the help of the enzyme ß-Hydroxybutyratedehydrogenase (HBDH). HBDH can catalyze the oxidative dehydrogenation of ß-Hydroxybutyrate. At the same time, NAD+ is reduced to NADH, which can be quantified spectrophotometrically at 340nm.

Purity

>90% by SDS-PAGE

Molecular weight

28kDa (experimentally determined molecular weight)

Storage

Store desiccated at -15°C or below. Allow to come to room temperature before opening. Before returning to storage, re-desiccate under vacuum for a minimum of four hours. Re-seal before returning to -15°C or below.

Reconstitution

Dissolves readily at 5mg/ml in 0.1M Tris/HCl, pH 8.5, to give a clear solution

Optimum pH/ temperature

pH 8.5, 37°C (0.1M Tris/HCl buffer)

Activity

Not less than 400U/mg material. (Unit definition: amount of enzyme causing the reduction of one micromole of NAD per minute at 37°C and pH 8.5)

Downloadable materials

HBDH Product Sheet (PDF)

Diabetes Brochure (PDF)

Product Specification Sheet (PDF)

HBDH Safety Data Sheet