Detect diabetic ketoacidosis faster and more reliably than traditional tests.
Introducing HBDH, our latest innovation for DKA test manufacturers.
HOW IT WORKS
Helping you create tests that measure what matters most.
Using cutting edge recombinant technology, our Hydroxybutyrate Dehydrogenase (HBDH) works by measuring ß-Hydroxybutyrate, the most abundant ketone that builds up during ketosis.
This is unlike traditional nitroprusside methods, which only react with acetoacetate and acetone. By using HBDH, you are able to detect DKA earlier and more accurately than ever before.
 
 
78
%
ß-Hydroxybutyrate
 
Acetoacetate (20%)
 
Acetone (2%)
 
With the silent epidemic of diabetes on the rise, the urgency to address diabetic ketoacidosis (DKA) cannot be overstated.

As a world leading manufacturer of critical materials for the diabetes market, BBI Solutions is proud to launch our new recombinant HBDH to address this global threat.
 
 
Proven Reliability
Our recombinant manufacturing process delivers best in class consistency, so you can trust in the efficacy of our HBDH in detecting DKA.
 
Consistent quality
As an ISO 13485 certified manufacturer you can rest assured you have access to a consistent high quality product, batch after batch, saving you time and resources.
 
5 Billion blood glucose test strips and counting
As the trusted component in over 5 billion tests for the most demanding, world-leading IVD manufacturers, our track record speaks for itself - as does our extensive portfolio of diabetes products.
 
From raw materials to results
As a global leader in the primary manufacture of diagnostic enzymes, we are a key player in the supply of critical raw materials for the diagnosis and management of diabetes.
 
Wraparound support as standard
Our experts support you in building customised assays and tests, ensuring the market success of your diagnostic solutions.
 
Investing in diabetes
Our new Centre of Excellence strengthens our recombinant manufacturing capabilities to fully cater to our customers and deliver a wider range of critical raw materials.
testimonial

BBI has a long-standing commitment to diabetes management and we’re excited to add HBDH to our extensive range. 

Customers will benefit from our new state-of-the-art Centre of Excellence which expands and upgrades our ability to cultivate and purify recombinant proteins like HBDH. 

This is supported, as always, by our team of world-leading experts at every stage, giving BBI customers an advantage in developing best-in-class end products. 

Mario Gualano
CEO, BBI Solutions
SUpport
Frequently Asked Questions
How does the recombinant HBDH need to be stored?
How can I reconstitute recombinant HBDH (rHBDH)?
What is the activity of recombinant HBDH?
Is the product available in bulk amounts?
What other diabetes products are in the BBI Portfolio?
Tech specs
D-3-Hydroxybutyrate Dehydrogenase (HBDH)
 
Alternative names
(R)-3- Hydroxybutanoate : NAD+ oxidoreductase
Product code
rHBDH
E.C number
1.1.1.30
CAS number
9028-38-0
Systematic name
NAD+ oxidoreductase
Source
Recombinant HBDH (Alcaligenes faecalis) expressed in E. Coli
Form
A freeze-dried material
Suggested applications
Recombinant enzyme to measure serum levels of ß-Hydroxybutyrate as a method to diagnose and monitor diabetic ketoacidosis (DKA) and its treatment.
Enzyme function
ß-Hydroxybutyrate is measured with the help of the enzyme ß-Hydroxybutyratedehydrogenase (HBDH). HBDH can catalyze the oxidative dehydrogenation of ß-Hydroxybutyrate. At the same time, NAD+ is reduced to NADH, which can be quantified spectrophotometrically at 340nm.
Purity
>90% by SDS-PAGE
Molecular weight
28kDa (experimentally determined molecular weight)
Storage
Store desiccated at -15°C or below. Allow to come to room temperature before opening. Before returning to storage, re-desiccate under vacuum for a minimum of four hours. Re-seal before returning to -15°C or below.
Reconstitution
Dissolves readily at 5mg/ml in 0.1M Tris/HCl, pH 8.5, to give a clear solution
Optimum pH/ temperature
pH 8.5, 37°C (0.1M Tris/HCl buffer)
Activity
Not less than 400U/mg material. (Unit definition: amount of enzyme causing the reduction of one micromole of NAD per minute at 37°C and pH 8.5)